Factor | Factor level |
---|---|
Ratios of DMSO | {40%, 50%, 60%} |
Thawing time (TT) | {1 h, 4 h, 7 h} |
Needle external diameter (NED) | {18G, 20G, 22G} |
Insertion velocity (IV) | { 1 mm/s, 3 mm/s, 6 mm/s } |
Repeated insertion times (RITs) | {1, 2, 3} |
Insertion positions (IP) | {5 mm, 10 mm} |